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Fields are generally the most important objects in any layout. You ve seen how to add fields at layout creation time. The initial default layout automatically places every field you ve defined for the database. When you design a new layout with the New Layout/Report Assistant, you specify which fields to place as well as the order in which they are placed. You can also add a field to any layout at any time, even if the field didn t exist when you created the layout. You add fields to an existing layout by selecting the Field tool, by choosing Insert Field, or by clicking the Insert Field button on the Tools toolbar (if you have the Tools toolbar visible). The Field tool is in the center of the Tools sidebar.

FIGURE 2.10 Anatomy of a panel. Panels contain a flyout menu that provides additional options. Floating panel groups also have a Close box to remove them from the display. Some panels have a hide/show options button to display and hide additional fields. Collapsed panels Expand/collapse button Close box Flyout menu Expand/collapse button

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Data visualization tools are far more abundant with every passing year. Many of these tools cropping up in recent years are compact Shockwave Flash (.swf) files capable of receiving and rendering arrays of user data. The variety and quantity of this class of tools is steadily increasing, and a number of the tools are available free (and others are licensed for modest shareware fees). The significance of this burgeoning technology for FileMaker lies in the opportunities you have to incorporate Flash charting into your solution. You can perform this feat by

Note: As soon as there is more than a single colony, clumps of the nascent hES cell line should be frozen at every passage until large numbers of cryo-straws from each cell line have been successfully frozen (see Section 2.6). Minimally 50% of the rst 10 15 passages should be frozen until the cell line is well-established.

Provided that the kinetic mechanism of a biocatalyst of interest obeys the irreversible, singlecatalytic-site, ordered Bi Bi kinetic scheme, then plots of actual 1/ versus 1/[SA ] data obtained in the presence of various xed concentrations of the SB substrate, [SB ], should obey the double-reciprocal equation (8.69) giving the putative graphical output shown in Figure 8.37. Equation (8.69) and this ordered Bi Bi kinetic scheme are applicable for the steady state kinetic analyses of several of the biocatalysts outlined in Section 8.1. These include porcine mitochondrial MDH and E. coli CAT (Figures 8.2 and 8.7, Table 8.1). In the case of MDH, the leading substrate, SA , is NAD+ and the following substrate, SB , is oxalate. MDH is homodimeric but both catalytic sites are completely independent. Clearly, Vmax f values derived from Equation (8.69) can be converted into kcat values for MDH that must be divided through by two in order to derive the correct turnover number per catalytic site. With respect to CAT, only one catalytic site binds both substrates but the mechanism may be random, in which

The third-generation Grid systems now under development are beginning to exhibit many of these features. In this section we consider rst the service-oriented approach, looking at Web services and agent-based computing, and then the information layer issues.

Collimator Choices custom made or adapted from available components. Let s stop for a moment s practical discussion of some of the options that confront this choice.

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